| Replacement Information | |
|---|---|
| Replacement Information | AB1541 is a recommended replacement for AB1556 |
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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | IHC, WB | Sh | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1541 |
| Replaces | AB1556 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Tryptophan Hydroxylase Antibody |
| Alternate Names |
|
| Background Information | Tryptophan hydroxylase (TPH) is an enzyme involved in the synthesis of the neurotransmitter serotonin. It is responsible for addition of the -OH group (hydroxylation) to the 5 position to form the amino acid 5-hydroxytryptophan (5-HTP), which is the initial and rate-limiting step in the synthesis of the hormone serotonin. It is also the first enzyme in the synthesis of melatonin. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified sheep polyclonal liquid in 150 mm NaCl, 10 mm HEPES (pH 7.5), 100 μg/mL BSA and 50% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Tryptophan Hydroxylase using this Anti-Tryptophan Hydroxylase Antibody validated for use in IH & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry(peroxidase): Performed on formaldehyde fixed tissue of a previous lot. Useful for cell body but not fiber staining. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Sheep |
| Specificity | Recognizes Tryptophan Hydroxylase. Reacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species. Recently studies have indicated that AB1541 can cross react with Tyrosine hydroxylase on western blots, weakly. Cross reactivity in the native state is untested. |
| Species Reactivity |
|
| Species Reactivity Note | Reacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the gut, pineal gland, spleen, and thymus. |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | COFACTOR:Fe(2+) ion. SIZE:444 amino acids; 50985 Da SUBUNIT:Multimer of identical subunits. TISSUE SPECIFICITY:Isoform 2 seems to be less widely expressed than isoform 1. SIMILARITY:SwissProt: P17752 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. & Contains 1 ACT domain. |
| Molecular Weight | 55 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on PC12 lysates. Western Blot Analysis: 1:1000 dilution of this lot detected Tryptophan Hydroxylase on 10 μg of PC12 lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at -20ºC to -80ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |