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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3565 |
| Replaces | 04-575; 04-576 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-PARP (214/215) cleavage site Antibody |
| Alternate Names |
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| Background Information | Poly (ADP-Ribose) Polymerase (PARP) is a 116 kDa nuclear protein which is strongly activated by binding to DNA strand breaks. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Dulbeccos phosphate buffered saline (without mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PARP (214/215) cleavage site Antibody detects level of PARP (214/215) cleavage site & has been published & validated for use in WB & IC. |
| Key Applications |
|
| Application Notes | For Western blot applications, we recommend using the antibody at a 1:1,000 starting dilution. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP. |
| Host | Rabbit |
| Specificity | The antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells. |
| Species Reactivity |
|
| Species Reactivity Note | Reactivity with other species has not been confirmed. This sequence is 86% homologous in mouse, rat, and hamster. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. |
| Gene Symbol |
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| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P09874 # Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. SIZE: 1014 amino acids; 113084 Da SUBUNIT: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with ZNF423 (By similarity). SUBCELLULAR LOCATION: Nucleus. PTM: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. & Poly-ADP-ribosylated by PARP2. SIMILARITY: SwissProt: P09874 ## Contains 1 BRCT domain. & Contains 1 PARP alpha-helical domain. & Contains 1 PARP catalytic domain. & Contains 2 PARP-type zinc fingers. MISCELLANEOUS: The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Purified from rabbit serum by affinity chromatography using a peptide corresponding to the PARP cleavage site. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |