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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3594-50UL |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Aquaporin 4 Antibody, CT |
| Alternate Names |
|
| Background Information | Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special proteins "aquaporins". Aquaporins, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. The water-specific channel Aquaporin 4 is located in various tissues including the gastrointestinal tract, kidney and brain. It is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. In CNS tissue, AQP4 localizes to astrocyte endfeet making it a useful structural marker. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Affinity Purified immunoglobulin. Amber vial of Rb X Aquaporin 4, C-Terminus (part # AB3594-50ULa), lyophilized from PBS, pH 7.4, containing 1% BSA, and 0.05% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min). |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Aquaporin 4 Antibody, C-terminus is validated for use in IH, WB for the detection of Aquaporin 4. |
| Key Applications |
|
| Application Notes | Western blotting: 1:500 on rat brain membranes. Immunohistochemistry Dilutions should be made using a carrier protein such as BSA (1-3%). Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | GST fusion protein with residues 249-323 of rat Aquaporin 4 (SwissProt accession number P47863). It is expected that the antibody may also react with mouse (73/75), bovine (71/75), human (69/75) and rabbit (64/75) due to sequence homology. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes aquaporin 4. On western blots of rat brain membranes, the antibody detects a band migrating at approximately 38 kDa corresponding to aquaporin 4. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P55087 # Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. SIZE: 323 amino acids; 34830 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Brain - muscle > heart, kidney, lung, and trachea. DOMAIN: SwissProt: P55087 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. |
| Molecular Weight | 38 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |