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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3768 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Rad54L Antibody |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 RPM) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a 1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25C° prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Rad54L Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Rad54L & has been validated in ICC & IHC. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:200-1:1,000. Immunocytochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user.SUGGESTED PROTOCOL FOR IMMUNOHISTOCHEMISTRY 1. Dissect tissues and freeze on dry ice 2. Cut on a cryostat - 10um slices 3. Dry slides at room temperature for 30-90 min 4. Fix with cold Acetone/Methanol (50/50) 2 min - then either process further or store at -20°C . Alternative is fixation with 4% PFA and treatment with a trypsin solution. (0.05% trypsin , incubation 10 minutes at 37°C, followed by 3 washes with PBS) 5. Let air dry 6. PBS 5min 7. Incubate in 50mM ammonium chloride 30 min 8. PBS 5min 9. Blocking serum 30-45min 10. Primary antibody 90min - dilution 1:100 - 1:600 11. Wash 3 times for 5 min 12. Secondary fluorescent conjug. antibody 30min 13. Wash 3 times for 5 min and mount |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide, amino acids 12-27 of RAD51L. |
| Host | Rabbit |
| Specificity | Recognizes RAD54L. The molecular weight of the protein is 84,326 Daltons. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q92698 # Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells. SIZE: 747 amino acids; 84352 Da SUBUNIT: Interacts with RAD51 through the NH2-terminal domain. SUBCELLULAR LOCATION: Nucleus. DISEASE:SwissProt: Q92698 # Defects in RAD54L may be a cause of tumor formation. Although alterations are found in a small fraction of primary tumors, these findings provide new insight into genetic instability underlying carcinogenesis. SIMILARITY: SwissProt: Q92698 ## Belongs to the SNF2/RAD54 helicase family. & Contains 1 helicase ATP-binding domain. & Contains 1 helicase C-terminal domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.The rehydrated antibody solutions can be stored undiluted at 2-8C° for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8C°. For storage at -20C°, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70C° for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |