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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ELISA, IHC, IH(P), WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3786 |
| Replaces | 07-647 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Prosurfactant Protein C (proSP-C) Antibody |
| Alternate Names |
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| Background Information | Pulmonary surfactant is a complex mixture of phospholipids and proteins secreted from airway epithelial cells (type II cells) in alveoli which reduces the surface tension at the alveolar air liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C and D. SP A (28 - 36 kDa) and SP D (43 kDa) are collagenous carbohydrate binding proteins, whereas SP B (8 - 9 kDa) and SP C (4 kDa) are non collagenous hydrophobic proteins. SP C is a fragment of a much larger precursor protein of 21 kDa. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Polyclonal rabbit serum containing 0.01% sodium azide as a preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Prosurfactant Protein C (proSP-C) Antibody detects level of Prosurfactant Protein C (proSP-C) and has been published & validated for use in ELISA, Immunohistochemistry (IHC), Immunohistochemistry - Paraffin (IHC-P) and Western Blotting (WB). |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:2,000-1:4,000 dilution of a previous lot was used on human tissue (see protocol), 1:4,000 dilution on adult mouse lung and 1:1,000-1:2,000 dilution on fetal mouse lung (see protocol). Immunohistochemistry: 1:2,000-1:4,000 on human tissue with and without antigen retrieval (see protocol) and 1:1,000-1:2,000 on adult and 1:500-1:1,000 on fetal mouse lung (see notes). Immunoblot: 1:5,000 (best if not reduced) Western Blot Analysis: a previous lot used a dilution of 1:500 ELISA: 1:50,000 IMMUNOHISTOCHEMISTRY : Notes: 1) Dilutions used are for normal (i.e. uninjured) adult mouse lung and have been used on FVB/N, Balb/c, nu/nu and SCID mice. The use of antigen retrieval systems to enhance immunostaining for this antibody is not necessary in the mouse lung and may actually increase background staining. Optimal dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | A synthetic peptide corresponding to amino acids 1-32 from the N-terminal of the human SP-C proprotein coupled to KLH |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Reacts strongly with proSP-C from human and mouse. Immunoprecipitates the 21 kDa proprotein as well as processing intermediates of M.W. 26 kDa, 21 kDa, 16 kDa and 14 kDa. Preincubation of the antiserum with the fusion protein results in ablation of immunostaining. On human tissue immunostains alveolar Type II cells in normal lung. The antibody also recognizes the aberrant form of proSP-C (6-12 kDa peptide) found in BAL and in alveolar proteinosis material on sections of lung samples from human patients with hereditary SP-B deficiency. On mouse tissue immunostains alveolar Type II cells in normal lung. |
| Species Reactivity |
|
| Species Reactivity Note | Mouse and rat. Expected to cross-react with human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The SFTPC gene encodes pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. It is produced exclusively by type II alveolar epithelial cells in the lung. Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. Surfactant is composed of phospholipids and other surfactant-associated proteins (Clark and Clark, 2005 [PubMed 15927881]). See also SFTPA1 (MIM 178630), SFTPB (MIM 178640), and SFTPD (MIM 178635).[supplied by OMIM] ... |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P11686 # Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. SIZE: 197 amino acids; 21053 Da SUBCELLULAR LOCATION: Secreted, extracellular space. DISEASE: SwissProt: P11686 # Polymorphisms in the SFTPC gene are important determinants for predisposition to respiratory distress syndrome (RDS) in premature infants [MIM:267450]. RDS in the newborn is the main cause of mortality and morbidity in premature infants. RDS is characterized by deficient gas exchange that is caused by diffuse atelectasis and high-permeability lung edema that results in fibrin-rich alveolar deposits called hyaline membranes. The risk of bronchopulmonary dysplasia increases with the severity of RDS. & Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. SMDP2 is a rare autosomal dominant disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course. The pathophysiology of the disorder is postulated to involve intracellular accumulation of a structurally defective SFTPC protein. Some patients who survive to adulthood manifest features of pulmonary fibrosis such as shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees in inflammation, fibrosis, or both on biopsy. Pulmonary surfactant metabolism disorders are genetically heterogeneous and associated with various pathologic entities, including pulmonary alveolar proteinosis (PAP), desquamative interstitial pneumonitis (DIP), or cellular non-specific interstitial pneumonitis (NSIP). & Genetic variations in SFTPC are associated with respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS in the newborn is the main cause of mortality and morbidity in premature infants. RDS is characterized by deficient gas exchange that is caused by diffuse atelectasis and high-permeability lung edema that results in fibrin-rich alveolar deposits called hyaline membranes. The risk of bronchopulmonary dysplasia increases with the severity of RDS. SIMILARITY: SwissProt: P11686 ## Contains 1 BRICHOS domain. MISCELLANEOUS: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C). |
| Molecular Weight | 21 kDa proprotein as well as processing intermediates of M.W. 26 kDa, 21 kDa, 16 kDa and 14 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on rat lung lysates. Western Blot Analysis: 1:500 dilution of this lot detected Prosurfactant Protein C on 10 μg of rat lung lysates |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable at -20ºC in undiluted aliquots for up to twelve months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |