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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, PO, Ca, Mk, Pm, Eq, B | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB6000 |
| Replaces | AB802 |
| Description | Anti-TIMP-3 Antibody, CT |
| Alternate Names |
|
| Background Information | Tissue inhibitor of metalloproteinases 3 (TIMP-3) is a member of the TIMP family. The TIMPs are SO named because they are slow, tight binding endogenous inhibitors of the Matrix Metalloproteinases (MMPs). The four TIMPs have different inhibition constants for the different MMPs studied. TIMPs have been shown to have activity against the ADAMs family of proteinases. TIMP-3 is an efficient "sheddase" inhibitor, inhibiting ADAM-17 (TACE) at the low nanomolar levels seen with MMP inhibition. The other ADAMs proteinases have not yet been assayed for TIMP inhibition, but TIMP-2 seems to be much less active on ADAM-17 than isTIMP-3. TIMP-3 is thought to be constitutively produced by many cell types and is inducible in others. The TIMP-3 localization differs from the other 3 TIMPs, and is thought to be primarily deposited into the extracellular matrix. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mm NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-TIMP-3 Antibody, C-terminus is validated for use in WB, IC for the detection of TIMP-3. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected TIMP-3 in C6 cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human TIMP-3 at the C-terminus. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | The antibody recognizes TIMP-3 at the C-terminus. Does not appear to cross react with TIMP-1 or TIMP-2. |
| Species Reactivity |
|
| Species Reactivity Note | Human and rat. Predicted to react with pig, canine, monkey, primate, equine, and bovine based on 100% sequence homology and mouse at 95% homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsbys fundus dystrophy. [provided by RefSeq] |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them. May form part of a tissue- specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. SIZE: 211 amino acids; 24145 Da SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. DISEASE: Defects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years. SIMILARITY: Belongs to the protease inhibitor I35 (TIMP) family. & Contains 1 NTR domain. |
| Molecular Weight | Bands may be observed for reduced protein bands at ~ 24 kDa (unglycosylated) and ~ 30 kDa (glycosylated). Additional TIMP-3 bands MAY be visible at 50 kDa (dimer), 12 kDa, and 15 kDa (breakdown products) and sample dependent. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot using an HL-60 cell lysate supplemented with PMA-conditioned media. Western Blot Analysis: 0.5 µg/mL of this antibody detected TIMP-3 on 10 µg of HL-60 in PMA-conditioned media. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |