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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB6004 |
| Replaces | AB815 |
| Description | Anti-MT1-MMP Antibody, hinge region |
| Alternate Names |
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| Background Information | Matrix metalloproteinases (MMPs) are a family of secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes degrade the components of extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. MMPs play an important role in wound healing, apoptosis, bone elongation, embryo development, angiogenesis, cancer metastases, and tissue remodeling within many disease states. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MT1-MMP (MMP-14) is a member of the membrane-type subfamily. Each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. MT1-MMP is capable of mediating pericellular proteolysis of extracellular matrix components and is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix. This protein also activates MMP2 protein, and this activity may be involved in tumor invasion. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified in 0.1M Tris-Glycine, pH 7.4, 150 mm NaCl with 0.05% NaN3. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect MT1-MMP using this Anti-MT1-MMP Antibody, hinge region validated for use in WB, IH(P). |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | KLH conjugated synthetic peptide selected from the hinge region of human MT1-MMP. |
| Epitope | Hinge region |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | The antibody recognizes human and rat MT1-MMP. It does not cross react with MMP-1, MMP-2, MMP-8, MMP-9, and MMP-13. |
| Species Reactivity |
|
| Species Reactivity Note | Human and rat. Predicted to cross react with mouse, (95% sequence homology) and monkey chimpanzee, canine and bovine (100% sequence homology). Reactivity with other species has not been tested. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Peptide affinity purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. CATALYTIC ACTIVITY: Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain. COFACTOR: Binds 1 zinc ion per subunit By similarity. Calcium By similarity. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein Potential. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck. DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. PTM: The precursor is cleaved by a furin endopeptidase By similarity. SEQUENCE SIMILARITIES: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. |
| Molecular Weight | ~ 65 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated on a representative lot by Western blot on rat lung lysate using Anti-MT1-MMP. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C for up to 1 year from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |