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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IP, WB, ICC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB9856 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-TorsinB Antibody |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Liquid. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect TorsinB using this Anti-TorsinB Antibody validated for use in IP, WB & IC. |
| Key Applications |
|
| Application Notes | Western blot: 1:100-1:200. The antibody reacts with the ~38 kDa protein. The suggested blocking buffer is TBS with 10% milk and 0.05% Tween-20. The suggested dilution buffer is TBS with 1% milk and 0.05% Tween-20. Note: TorsinB is a glycoprotein which can be deglycosylated by either EndoH or PNGase treatment. Immunocytochemistry: 1:100 using rhodamine conjugated goat anti-rabbit secondary antibody. Suggested fixative is 4% paraformaldehyde fixed (37°C for 20 minutes) cells. Suggested blocking solution should contain 10% goat serum. Suggested dilution buffer is PBS containing 1% BSA. Immunoprecipitation. Recommended antibody amount is 5-7 mL of rabbit serum. The preferred tissue/cell lysis buffer is RIPA. Final reaction volume of 1000 mL. Final total protein concentration in reaction mix: 0.1-0.5 mg/mL. Suggested incubation time is 16-18 hours at 2-8°C. Sugggested capture is Protein A/G. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide from torsinB. |
| Host | Rabbit |
| Specificity | TorsinB. The antibody reacts with the ~38 kDa protein. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Serum |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: O14657 # May serve as a molecular chaperone assisting in the proper folding of secreted and/or membrane proteins (By similarity). SIZE: 336 amino acids; 37979 Da SUBCELLULAR LOCATION: Endoplasmic reticulum lumen (By similarity). TISSUE SPECIFICITY: Widely expressed with low levels in brain. Not detected in fetal brain. SIMILARITY: SwissProt: O14657 ## Belongs to the clpA/clpB family. Torsin subfamily. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |