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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | IHC, WB | Rb | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB9864 |
| Replaces | AB1516; 07-362 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-NMDAR1 Antibody, rabbit monoclonal |
| Alternate Names |
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| Background Information | N-methyl-D-aspartate Receptors (NMDAR) are one of three pharmacologically distinct subtypes of ionotropic receptors that mediate a majority of excitatory neurotransmission in the brain via the endogenous amino acid, L-glutamate. MDARs form heteromeric complexes usually composed of two components, the NR1 subunit and NR2 subunits. Both proteins are weakly expressed throughout the brain but show localization in the dendritic region of the mushroom bodies. The NMDAR channel is highly permeable to Ca2+ and Na+ and its opening requires the simultaneous binding of glutamate and postsynaptic membrane depolarization. The activated NMDAR channel allows calcium influx into the post-synaptic cell where calcium triggers a cascade of biochemical events that create synaptic changes. NMDAR have been studied extensively in Drosophila for its potential relationship to behavioral plasticity. A newly identified NMDAR complex of more than 80 different proteins has been identified. Genetic and pharmacological disruptions of components of the NMDAR complex produce learning impairments in rodents, further linking behavioral plasticity to synaptic plasticity. |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Control |
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| Presentation | Rabbit monoclonal supernatant liquid containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-NMDAR1 Antibody is validated for use in IH, WB for the detection of NMDAR1. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: AB9864 can be used for using 4% paraformaldehyde or paraformaldehyde/glutaraldehyde fixed rat tissue. Suggested working dilution for light microscopy is 1:1,000-1:2,000. Western blot: 1:1,000-1:2,000 using ECL on rat brain lysate. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to the C-terminus of rat NMDA receptor subunit (Catalog number AG344). {amino acids 909-938 rat NMDAR1{LQNQKDTVLPRRAIERE EGQLQLCSRHRES} |
| Epitope | a.a. 909-938 |
| Clone | 1.17.2.6 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | The original sequence used is selective for splice variants NR1-1a, NR1-1b, NR1-2a, NR1-2b using the nomenclature defined by Hollmann, M. et Al. (1993). These appear to be the major splice variants expressed in rat brain (Sugihara H, et Al., 1992). No cross-reaction with other glutamate receptor subunits. Western Blot analysis shows that this rabbit monoclonal antibody labels a single band at approximately 120 kD corresponding to NMDAR1 in rat brain lysate. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Reactivity in mouse is weak. On reduced Western blots a band matching that in rat is observed but tests in immunohistochemistry have been unsuccessful. It is expected that the antibody will also react with monkey, gerbil, rabbit, feline and fish based on the fact that another antibody made to the same peptide sequence reacts with those species. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q05586 # NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors (By similarity). SIZE: 938 amino acids; 105373 Da SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B, GRIN3A or GRIN3B and PPP2CB. Interacts with DLG4 and MPDZ (By similarity). SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Note=Enriched in post-synaptic plasma membrane and post-synaptic densities (By similarity). PTM: NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity (By similarity). SIMILARITY: SwissProt: Q05586 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family |
| Molecular Weight | 120 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on rat brain lysates. Western Blot Analysis: 1:1000 dilution of this lot detected NMDAR1 on 10 μg of rat brain lysates |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |