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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | IP, WB, ICC, IHC | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB9876 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Stargazin Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid in 130 mM Tris-Cl, 87 mM Glycine, pH 7.4. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Stargazin Antibody is validated for use in IP, WB, IC, IH for the detection of Stargazin. |
| Key Applications |
|
| Application Notes | Western blot: 0.1 μg/mL on Triton soluble fraction of rat brain. Suggested dilution buffer is TBS-T and blocking buffer is 3% skim milk. Suggested incubation time is 3 hours to overnight at room temperature. Immunohistochemistry: 0.25-0.5 μg/mL on 4% paraformaldehyde fixed tissue. Suggested dilution buffer is PBS-T. Suggested permeabilization method is 0.1% Triton X-100. Suggested incubation time is 2 hours to overnight at room temperature. Immunocytochemistry Immunoprecipitation: 1-2 μg. Suggested reaction volume is 200-1000 μL. Suggested total protein concentration is 2 mg/mL. Suggested incubation time is 2 hours at 2-8°C. Suggested capture agent is Protein A. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | GST fusion protein from rat stargazin, cytoplasmic domain. |
| Host | Rabbit |
| Specificity | Stargazin [Stargazin-like TARP (transmembrane AMPA receptor regulatory protein)]. By Western blot the antibody recognizes bands at ~35 kDa and ~55 kDa on Triton soluble fraction of rat brain. The antibody reacts dominantly with TARP gamma-8 and weakly with gamma-3 and gamma-4. Stargazin is expressed strongly in the hippocampus and cerebellum. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | L-type calcium channels are composed of five subunits. The protein encoded by this gene represents one of these subunits, gamma, and is one of several gamma subunit proteins. It is an integral membrane protein that is thought to stabilize the calcium channel in an inactive (closed) state. This protein is similar to the mouse stargazin protein, mutations in which have been associated with absence seizures, also known as petit-mal or spike-wave seizures. This gene is a member of the neuronal calcium channel gamma subunit gene subfamily of the PMP-22/EMP/MP20 family. |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q9Y698 # Thought to stabilize the calcium channel in an inactivated (closed) state. SIZE: 323 amino acids; 35966 Da SUBUNIT: The L-type calcium channel is composed of five subunits: alpha-1, alpha-2/delta, beta and gamma. Interacts with the PDZ domains of DLG4/PSD-95 and DLG1/SAP97. May interact with GOPC (By similarity). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Brain. PTM: Phosphorylation of Thr-321 impairs interaction with DLG1 and DLG4. SIMILARITY: SwissProt: Q9Y698 ## Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -80°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze thaw cycles. The antibody may be kept at 2-8°C for 1-3 weeks after thawing. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |