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Millipore Anti-Ido Antibody Detects Level Of Ido & Has Been Published & Validated For Use In Elisa & Wb. - Mill (Additional S&H or Hazmat Fees May Apply)
Anti-IDO Antibody detects level of IDO & has been published & validated for use in ELISA & WB.
Key Applications
ELISA
Western Blotting
Application Notes
Western blot: 1:2,000-1:5,000 using ECL. Reacts with the ~45 kDa IDO protein. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature.
ELISA, direct and indirect: 1:2,000-1:10,000.
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
Recombinant mouse IDO.
Host
Rabbit
Specificity
IDO [Indoleamine 2,3-dioxygenase], mouse.
Species Reactivity
Mouse
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_002164.4
Entrez Gene Summary
Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]
Gene Symbol
INDO
IDO
EC 1.13.11.52
UniProt Number
P14902
UniProt Summary
FUNCTION: SwissProt: P14902 # Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. COFACTOR: Binds 1 heme group per subunit. SIZE: 403 amino acids; 45326 Da SIMILARITY: SwissProt: P14902 ## Belongs to the indoleamine 2,3-dioxygenase family.
Molecular Weight
45 kDa
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
