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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC299 |
| Description | Anti-FFAR2 Antibody/GPR43 |
| Alternate Names |
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| Background Information | Free fatty acid receptor 2 (UniProt: O15552; also known as G-protein coupled receptor 43) is encoded by the FFAR2 (also known as FFA2, GPCR43, GPR43) gene (Gene ID: 2867) in human. FFAR2 is a multi-pass membrane glycoprotein with four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. It is expressed at relatively high levels in peripheral blood leukocytes and at lower levels in the spleen. It is a G-protein-coupled receptor that is activated by short chain fatty acids (SCFAs) and plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, SCFAs containing less than 6 carbons, such as acetate, propionate, and butyrate, produced by the gut microbiome, are the most potent activators. These SCFAs not only serve as a source of energy, but also act as signaling molecules. The activation of this G-protein-coupled receptor leads to the formation of inositol 1,4,5-trisphosphate leading to the mobilization of intracellular calcium and phosphorylation of ERK1 and ERK2. It also plays a role in glucose homeostasis by regulating the secretion of GLP-1. It is reported to regulate whole-body energy homeostasis through adipogenesis regulating both differentiation and lipid storage of adipocytes. In parallel, FFAR2 also mediates the activation of the inflammatory chemokines and cytokines. FFAR2 is also involved in maintaining healthy gut microbiome and suppress intestinal carcinogenesis and its down modulation has been linked to colon cancers. (Ref.: Sivaprakasam, S., et al. (2016). Oncogenesis. 5; 238). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-FFAR2/GPR43, Cat. No. ABC299, is a rabbit polyclonal antibody that detects FFAR2/GPR43 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Tested Applications Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FFAR2/GPR43 in mouse kidney and mouse liver tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to 54 amino acids from the C-terminal region of human Free fatty acid receptor 2 (FFAR2). |
| Epitope | Cytoplasmic domain |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects Free fatty acid receptor 2 (FFAR2). It targets an epitope within the C-terminal region. |
| Species Reactivity |
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| Species Reactivity Note | Mouse. Predicted to react with Human, Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~50 kDa observed; 37.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in RAW264.7 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected FFAR2/GPR43 in RAW264.7 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |