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Millipore This Anti-Phospho-Cyld Antibody (Ser418) Is Validated For Use In Western Blotting For The Detection Of Phospho-Cyld (Ser418). - Mill (Additional S&H or Hazmat Fees May Apply)
CYLD is a deubiquitinating enzyme that inactivates transcription factor NF-kappaB using TNFRs and is essential for normal cellular homeostasis of skin appendages. Deactivation or mutation of CYLD causes a loss of the deubiquitination activity and increases tumorigenesis. Loss of CYLD specifically results in familial cylindromatosis which is characterized by a predisposition to cylindromas, tumors of skin appendages. CYLD has been shown to cleave the lysine 63-linked polyubiquitin chain from proteins and the inactivity of CYLD has also been noticed in many other human cancers.
Product Information
Format
Serum
Presentation
Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-phospho-CYLD Antibody (Ser418) is validated for use in Western Blotting for the detection of phospho-CYLD (Ser418).
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-CYLD (Ser418) in 10 µg of human spleen and Jurkat lysate. Western Blotting Analysis: A representative lot detected phospho-CYLD (Ser418) in PMA and Ionomycin-treated Jurkat cell lysate (Reiley, W., et al. (2005). Mole. Cell Biol. 25(10):3886-3895).
Biological Information
Immunogen
Linear peptide corresponding to human phospho-CYLD (Ser418).
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This antibody detects phospho-CYLD at Ser418. The phosphorylation site of CYLD shares some sequence homology with serine phosphorylation site of c-Cbl.
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001035814
Gene Symbol
CYLD
CYLD1
KIAA0849
HSPC057
Purification Method
Unpurified
UniProt Number
Q9NQC7
Molecular Weight
~110 kDa observed. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-CYLD (Ser418) in 10 µg of Raji cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
