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Millipore This Anti-Dishevelled Antibody Is Validated For Use In Western Blotting And Immunocytochemistry For The Detection Of Dishevelled. - Mill (Additional S&H or Hazmat Fees May Apply)
Dishevelled (DVL1), the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation. The protein acts as a transducer molecule for developmental processes, including segmentation and neuroblast specification. Dishevelled plays a role both in canonical and non-canonical Wnt signaling. DVL1 mutation is likely responsible for transformation in neuroblastomas, and the Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1 gene, thus Dishevelled maybe important in these diseases as well.
This Anti-Dishevelled Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Dishevelled.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in PC12 cells. (Kishida et al., 2007. Dvl regulates endo- and exocytotic processes through binding to synaptotagmin. Genes to Cells, 12, 49–61).
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to Human Dishevelled.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
Recognizes Human, Mouse and Rat Dishevelled 1, 2 and 3.
Species Reactivity
Human
Mouse
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_004412
Gene Symbol
DVL1
DVL2
DVL3
Purification Method
Unpurified
UniProt Number
O14640
Molecular Weight
~ 81 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HEK293T cell lysate.
Western Blot Analysis: A 1:1000 of this antibody detected Dishevelled in 10 µg of HEK293T cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
