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Millipore This Anti-Achaete-Scute Homolog 1 Antibody (Ascl1) Is Validated For Use In Western Blotting And Immunohistochemistry For The Detection Of Achaete-Scute Homolog 1 (Ascl1). - Mill (Additional S&H or Hazmat Fees May Apply)
Achaete-scute homolog 1 (ASCL1), also known as MASH1, ASH1 or HASH1, is a basic helix-loop-helix transcription factor. ASCL1 binds to the E box (5-CANNTG-3) which then activates transcription. For efficient DNA binding, dimerization with other BHLH proteins is required. Achaete Scute proteins may also be involved in the determination of neuronal precursors in the central and peripheral nervous systems. It is expressed in fetal brain and is essential for the proper development and the survival of subsets of autonomic neurons. It is highly expressed in medullary thyroid cancer and small cell lung cancer.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Achaete-scute homolog 1 Antibody (ASCL1) is validated for use in Western Blotting and Immunohistochemistry for the detection of Achaete-scute homolog 1 (ASCL1).
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Achaete-scute homolog 1 (ASCL1) in 5 µg of mouse E13-14 brain lysate and A549 cell lysate. Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Achaete-scute homolog 1 (ASCL1) in rat cerebellum, rat cerebral cortex, and rat hippocampus tissue.
Biological Information
Immunogen
BCP-conjugated linear peptide corresponding to human Achaete-scute homolog 1 (ASCL1).
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_004307
Gene Symbol
ASCL1
ASH1
BHLHA46
HASH1
Purification Method
Affinity Purfied
UniProt Number
P50553
Molecular Weight
~38 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting using lysate from cells over expressing Achaete-scute homolog 1 (ASCL1).
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Achaete-scute homolog 1 (ASCL1) in 5 µg of lysate from cells over expressing Achaete-scute homolog 1 (ASCL1).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
