Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE103 |
| Description | Anti-SIRT7 Antibody |
| Alternate Names |
|
| Background Information | The silent information regulator (SIR) genes (sirtuins) are a highly conserved family of proteins, which contain one or more sirtuins present in various species from bacteria to mammals. There are seven sirtuin genes that encode seven sirtuin enzymes, SIRT1 through SIRT7, all of which are dependent on oxidized nicotinamide adenine dinucleotide (NAD+). These proteins serve as mono-ADP-ribosyltransferases and deacetylate histones as a means of silencing specific chromatin regions. Further research is still required to elicidate the link between its effects on cellular metabolism, stress resistance, and cellular proliferation and its proposed role in mediating general lifespan. It is reported that Sirt7 expression demonstrates an anti-proliferative role with tumorigenic potential, in several murine cell lines. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-SIRT7 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect SIRT7 also known as NAD-dependent deacetylase sirtuin-7, SIR2-like protein 7. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:00 dilution from a representative lot detected the expressed protein of SIRT7 in human liver tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the N-terminus of the expressed protein of human SIRT7. |
| Epitope | N-terminus. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the N-terminus of the expressed protein of SIRT7. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: NAD-dependent protein deacetylase. Required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis. Promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT LOCATION: Interacts with UBTF and the RNA polymerase I complex. Interacts with histone H2A and/or histone H2B. SUBCELLULAR LOCATION: Cytoplasm. Nucleus > nucleolus. Note: Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis. |
| Molecular Weight | ~45 kDa observed. An additional isoform at ~20 kDa is known to exist. An uncharacterized band at ~30 kDa has been observed in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human liver tissue lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected the expressed protein of SIRT7 in 10 µg of human liver tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |