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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Chp | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE114 |
| Description | Anti-PRDM5 Antibody |
| Alternate Names |
|
| Background Information | PRDM5 is a member of the PR-domain containing family of tumor suppressors. PRDM5 activates histone methyltransferase G9a and class I histone deacetylases to regulate transcription. PRDM5 typically functions as a transcriptional repressor but can also be an activator for endogenous target genes. PRDM5 has often been found to be silenced in human breast, ovarian and liver cancers and and is a candidate biomarker for certain cancers, such as gastrointestinal cancer. Additional studies have also linked the loss of PRDM5 function to the development and pathogenesis of cervical cancer. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-PRDM5 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect PRDM5 also known as PR domain-containing protein 5. |
| Key Applications |
|
| Application Notes | Western Blot Analysis:1 µg/mL from a representative lot detected PRDM5 on 10 µg of HeLa cell lysate and HeLa nuclear extract. |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to human PRDM5. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Predicted to react with Chimpanzee based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Sequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes. SUBUNIT STRUCTURE: Interacts with EHMT2/G9A, GFI1 and HDAC1. SUBCELLULAR LOCATION: Nucleus SEQUENCE SIMILARITIES: Contains 16 C2H2-type zinc fingers. Contains 1 SET domain. |
| Molecular Weight | ~75 kDa observed. Three isoforms at 73, 70, and 13 kDa have been described. Uncharacterized bands between 13 and 73 kDa may be observed in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in PC3 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected PRDM5 on 10 µg of PC3 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |