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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ChIP, ICC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1391 |
| Description | Anti-Brd4 Antibody |
| Alternate Names |
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| Background Information | Bromodomain-containing protein 4 (Brd4), or Mitotic chromosome-associated protein (MCAP), is a chromatin reader protein and kinase that recognizes and binds acetylated histones and plays a key role in the transmission of epigenetic memory across cell divisions and transcription regulation. Brd4 remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for post mitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, Brd4 plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Brd4 is widely expressed and localized to the nucleus and acetylated chromatin. Brd4 also plays a large role in many cancers, and through its regulation of transcription, Brd4 is important in multiple cellular processes including DNA damage responses, aging, and development. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal serum containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Brd4 Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and Immunocytochemistry for the detection of Brd4. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot of this antibody was reported to detect Brd4 protein in a HeLa cell lysate (Alsarraj J., et al. (2013). Also see Anup, D., et al. (2003) and Mochizuki, K., et al. (2008) for additional data using mouse embyronic fibroblasts and NIH3T3 cells. Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to work in ChIP using chromatin from NIH3T3 cells. See Mochizuki, K., et al. (2008) and Anup, D., et al. (2009). Immunocytochemistry: A representative lot of this antibody was reported to work in ICC. See Anup, D., et al. (2000) and Anup, D., et al. (2009). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to Mouse Brd4 short form. |
| Epitope | Region between amino acids 150 to 290 of human Brd4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Recognizes human Brd4 |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with mouse based on sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~ 100 kDa observed This antibody primarily detects 100 kd short form of Brd4 and to lesser extent larger 180 kD form (see Alsarraj J., et al. (2013 for additional details on performance of this antibody). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cells. Western Blotting Analysis: 1:1,000 dilution of this antibody detected Brd4 in 10 µg of HEK293 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |