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Millipore This Anti-Mafa Antibody Is Validated For Use In Western Blotting And Immunocytochemistry For The Detection Of Mafa. - Mill (Additional S&H or Hazmat Fees May Apply)
V-maf musculoaponeurotic fibrosarcoma oncogene homolog A
Background Information
MAFA is a tissue specific transcription factor found in the nuclei of pancreas islet beta cells. MAFA specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. MAFA also cooperates synergistically with NEUROD1 and PDX1 in enhancing additional gene expression during development as well. Phosphorylation by GSK3 increases MAFA transcriptional activity and is required for its oncogenic activity. MAFA can be either an oncogene or act as a tumor suppressor, depending on the cell context.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-MAFA Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of MAFA.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot was reported to detect MAFA in HeLa, 293T/17, Capan-2, SAOS-2, SH-SY5Y, and Skin 3, 44 cells.
Biological Information
Immunogen
Mixture of three linear peptides corresponding to amino acids 1-150 of human MAFA.
Epitope
Region near the N-terminus of MAFA
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
Recognizes MAFA
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_963883
Gene Symbol
MAFA
Purification Method
Antigen Affinity Purified
UniProt Number
Q8NHW3
Molecular Weight
~ 35 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in a HEK293T cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected MAFA in a 293T cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
