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Millipore This Anti-Meiob Antibody Is Validated For Use In Western Blotting And Immunoprecipitation And Immunocytochemistry For The Detection Of Meiob. - Mill (Additional S&H or Hazmat Fees May Apply)
Meiosis-specific with OB domain-containing protein
Protein expressed in male leptotene and zygotene spermatocytes 675
MLZ-675
Background Information
MEIOB is a single stranded binding protein required for proper homologous recombination in meiosis I. MEIOB is required for proper DNA strand repair at double strand breaks and faithful synapsis of the chromosomes. MEIOB is also required for successful completion of meiotic recombination and is part of a multiprotein complex with RPA2 and SPATA22 proteins. MEIOB is expressed in the gonads and specifically in ovary and testis starting at the 14th weeks post fertilization. MEIOB is also expressed in a variety of tumors but not in other tissues other than testis and ovary. Because of its unique expression MEIOB is being examined as a possible anti-cancer autoimmune agent.
Product Information
Format
Serum
Presentation
Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-MEIOB Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of MEIOB.
Key Applications
Western Blotting
Immunoprecipitation
Immunocytochemistry
Application Notes
Immunoprecipitation Analysis: A representative lot immunoprecipitated MEIOB in P18 testes (Luo, M., et al. (2013). Nature. DOI: 10.1038/ncomms3788 pg. 1-12). Immunocytochemistry Analysis: A representative lot detected MEIOB in mouse spermatocytes (Luo, M., et al. (2013). Nature. DOI: 10.1038/ncomms3788 pg. 1-12).
Biological Information
Immunogen
Recombinant protein corresponding to mouse MEIOB.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Mouse
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_083473
Gene Symbol
Meiob
Purification Method
Unpurified
UniProt Number
Q9D513
Molecular Weight
~50 kDa observed. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse testis tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MEIOB in 10 µg of mouse testis tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
