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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE203 |
| Description | Anti-JARID1D Antibody |
| Alternate Names |
|
| Background Information | Also known as Lysine-specific demethylase 5D, this histone demethylase is specific for Lys-4 of histone H3 and is known to play a central role in histone function and spermatogenesis. It demethylates trimethylated and dimethylated, but not monomethylated H3 Lys-4. Uniprot.org states two different isoforms of this enzyme. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-JARID1D Antibody (rabbit polyclonal antibody) validated in WB, ICC, IP to detect JARID1D also known as Lysine-specific demethylase 5D, Histocompatibility Y antigen, H-Y, Histone demethylase JARID1D. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected JARID1D in mESCs. Immunoprecipitation Analysis: A representative lot immunoprecipitated JARID1D in human testis tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human JARID1D. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a protein containing zinc finger domains. A short peptide derived from this protein is a minor histocompatibility antigen which can lead to graft rejection of male donor cells in a female recipient. Alternative splicing results in multiple transcript variants. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Histone demethylase that specifically demethylates Lys-4 of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 Lys-9, H3 Lys-27, H3 Lys-36, H3 Lys-79 or H4 Lys-20. Demethylates trimethylated and dimethylated but not monomethylated H3 Lys-4. May play a role in spermatogenesis. COFACTOR: Ascorbate. Binds 1 Fe2+ ion per subunit. SUBUNIT STRUCTURE: Interacts with PCGF6 and MSH5. SUBCELLULAR LOCATION: Nucleus. |
| Molecular Weight | ~174 kDa observed. Two isoforms may be observed due to alternative splicing at a molecular weight of ~167 kDa and ~174 kDa in some cell lysates. An uncharacterized band at a molecular weight of ~255 kDa may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human testis tissue lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected JARID1D in 10 µg of human testis tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |