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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE62 |
| Description | Anti-MYSM1/2A-DUB Antibody |
| Alternate Names |
|
| Background Information | Histone H2A deubiquitinase MYSM, often referred to as 2A-DUB is metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A. 2A-DUB regulates transcription by coordinating histone acetylation and deubiquitination, and destabilizing the association of linker histone H1 with nucleosomes. The 2A-DUB mechanism may just be one of many H2A ubiquitination proteins that have the ability to regulate transcription. 2A-DUB is also essential for cell cycle progression and gene expression. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit polyclonal serum containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MYSM1/2A-DUB Antibody is a Rabbit Polyclonal Antibody for detection of MYSM1/2A-DUB also known as Histone H2A deubiquitinase MYSM1 & has been validated in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human of MYSM1. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. SUBUNIT STRUCTURE: Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Binds histones. SUBCELLULAR LOCATION: Nucleus DOMAIN: Binds double-stranded DNA via the SANT domain. The SWIRM domain does not bind double-stranded DNA. POST-TRANSLATIONAL MODIFICATION: Phosphorylated upon DNA damage, probably by ATM or ATR. SEQUENCE SIMILARITIES: Belongs to the peptidase M67A family. MYSM1 subfamily. Contains 1 MPN (JAB/Mov34) domain. Contains 1 SANT domain. Contains 1 SWIRM domain. SEQUENCE CAUTION: The sequence BAB67808.1 differs from that shown. Reason: Erroneous initiation. The sequence BAG54377.1 differs from that shown. Reason: Erroneous initiation. |
| Molecular Weight | ~100 kDa observed. Uncharacterized bands at ~26, ~35, ~49 and ~66 kDa may be observed in in some lysates. Bands at ~66 and ~26 kDa may represent isoforms resulting from alternative splicing. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in NIH/3T3 cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected MYSM1 on 10 µg of NIH/3T3 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |