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Millipore Detect Otu Domain-Containing Protein 4 Using This Rabbit Polyclonal Antibody, Anti-Otud4 Antibody Validated For Use In Western Blotting, Icc & Immunofluorescence. - Mill (Additional S&H or Hazmat Fees May Apply)
OTU domain-containing protein 4 (OTUD4) is a member of the OTU (ovarian tumor) domain containing cysteine protease superfamily, in which the OTU domain generally confers deubiquitinase activity. High levels of OTUD4 are found in lung, ovary, testis, liver, heart skeletal muscle, kidney, and brain, with lower levels present in pancreas and spleen. Four isoforms of OTUD4 are known to exist, the shortest one being expressed specifically in HIV-1-infected cells.
Product Information
Format
Affinity Purified
Control
Daudi cell lysate
Presentation
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Detect OTU domain-containing protein 4 using this rabbit polyclonal antibody, Anti-OTUD4 Antibody validated for use in western blotting, ICC & Immunofluorescence.
Key Applications
Western Blotting
Immunocytochemistry
Immunofluorescence
Application Notes
Immunocytochemistry Analysis: 2.5 µg/mL from a representative lot detected OTUD4 in Daudi cells.
Immunofluorescence Analysis: 20 µg/mL from a representative lot detected OTUD4 in Daudi cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human OTUD4.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Gene Symbol
OTUD4
HIN1
Purification Method
Antigen Affinity Purified
UniProt Number
Q01804
Molecular Weight
~108 kDa observed. Uniprot describes four isoforms at ~17 kDa, ~24 kDa, ~117 kDa, and ~124 kDa. Uncharacterized bands may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Daudi cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected OTUD4 in 15 µg of Daudi cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
