The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies.Learn about our cookie policy.
Millipore This Cytochrome P450 (Scc) Antibody Is Validated For Use In Wb & Ihc For The Detection Of The Cytochrome P450 (Scc) Protein. - Mill (Additional S&H or Hazmat Fees May Apply)
As a member of the cytochrome P450 family, Cytochrome P450 Side Chain Cleavage Enzyme (scc) catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone during the first step of steroid biosynthesis. It is expressed in certain organs, such as ovaries, adrenal glands, and testis. Deficiency in CYP11A1 is commonly associated with adrenal insufficiency, and with variable degrees of disorder of sex development (DSD).
Product Information
Format
Affinity Purified
Control
Rat adrenal tissue lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Cytochrome P450 (scc) antibody is validated for use in WB & IHC for the detection of the Cytochrome P450 (scc) protein.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:4,000-8,000 dilution from a representative lot detected Cytochrome P450 (scc) in rat adrenal gland and rat testis tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of rat Cytochrome P450 (scc), a.a. 509-526.
Epitope
C-terminus
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes the C-terminus of Cytochrome P450 (scc), a.a. 509-526.
Species Reactivity
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_058982
Gene Symbol
Cyp11a1
Cyp11a
Cyp11a-1
Purification Method
Affinity Purfied
UniProt Number
P14137
Molecular Weight
~52 kDa observed. On higher percentage gels and longer exposures, 8 kDa cleavage product can be detected.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in rat adrenal tissue lysate.
Western Blot Analysis: 0.02 µg/mL of this antibody detected Cytochrome P450 (scc) in 10 µg of rat adrenal tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
