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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS82 |
| Replaces | 07-1376 |
| Description | Anti-VEGF Antibody |
| Alternate Names |
|
| Background Information | Vascular endothelial growth factor A (UniProt: P15692; also known as VEGF-A, Vascular permeability factor, VPF) is encoded by the VEGFA (also known as VEGF) gene (Gene ID: 7422) in human. VEGF is a disulfide-linked homodimeric protein that acts as one of the most potent angiogenic cytokines to regulate vascular proliferation and permeability. It also acts as an anti-apoptotic factor for newly formed blood vessels. It is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to generate the mature form. Alternative splicing of a single VEGF gene generates five isoforms known as VEGF 121, 145, 165, 189 and 206, which differ in their molecular weights and in their ability to bind to cell-surface heparan sulfate proteoglycans. VEGF121 and VEGF165 are reported to be the most dominant forms expressed in all tumor cell lines investigated. VEGF121 is acidic and is freely secreted. However, VEGF165 is more basic, has heparin-binding properties and, although a significant proportion remains cell-associated, most is freely secreted. The biological effects of VEGF are mediated through two VEGF receptors, VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1), whose expression is largely, limited to the vascular endothelium. VEGF expression is enhanced in response to hypoxia, oncogenes, and other cytokines, and is associated with poor prognosis in several types of cancer. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-VEGF, Cat. No. ABS82, is a rabbit polyclonal antibody that detects Vascular endothelial growth factor (VEGF) and is tested for use in Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Tested Applications Immunohistochemistry (Paraffin) Analysis: A 1:200 dilution from a representative lot detected VEGF in human placenta tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human vascular endothelial growth factor A (VEGF-A). |
| Epitope | N-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes VEGF at the N-terminus. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Primate based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity). SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. ALTERNATIVE PRODUCTS: 10 named isoforms [FASTA] produced by alternative splicing. |
| Molecular Weight | ~ 57 kDa observed; 17.22 to 45.46 kDa calculated for various isoforms. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Human umbilical vein endothelial cells (HUVEC) lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected VEGF in Human umbilical vein endothelial cells (HUVEC) lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |