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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT179 |
| Description | Anti-Pro-neuregulin-1, membrane-bound isoform (NRG1 Antibody) |
| Alternate Names |
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| Background Information | Neuregulin isoforms have been classified as type I NRGs (isoforms with an Ig domain and a glycosylation domain, isoforms 1-8), type II NRGs (isoforms with an Ig domain but no glycosylation domain, isoform 9). Neuregulin Type I/II serve as direct ligands for ERBB3 and ERBB4 tyrosine kinase receptors that regulate development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Type I isoforms are the predominant forms expressed in the endocardium. Type II is the major form in skeletal muscle cells, and is expressed in the spinal cord, brain, adult heart, placenta, lung, liver, kidney, and pancreas. Neuregulin-HBD refers to the heparin binding domain of Type I/II Neuregulin. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Pro-neuregulin-1, membrane-bound isoform (NRG1 Antibody) is validated for use in Western Blotting and Immunohistochemistry for the detection of Pro-neuregulin-1, membrane-bound isoform (NRG1). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Pro-neuregulin-1, membrane-bound isoform (NRG1) in human cardiac myocytes and human cerebellum tissue. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to the extracellular domain of human Pro-neuregulin-1, membrane-bound isoform (NRG1). |
| Epitope | Extracellular domain |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This antibody is expected to cross-react to all Alpha and Beta isoforms (Alpha1A, Alpha2B, Alpha3; and Beta1/1A, Beta2, Beta3), also known as Type I. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Chimpanzee based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~110 and ~58 kDa observed. May detect multiple isoforms (Alpha1A, Alpha2B, Alpha3; and Beta1/1A, Beta2, Beta3). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in PC3 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pro-neuregulin-1, membrane-bound isoform (NRG1) in 10 µg of PC3 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |