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| Description | |
|---|---|
| Catalogue Number | AG220 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Choline Acetyltransferase, for Blocking AB143, AB144P Antibodies |
| Overview | Recombinant rat ChAT |
| Alternate Names |
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| Product Information | |
|---|---|
| Presentation | Liquid in 50 mm KPO4 buffer pH 7.5 containing 10% glycerol and 0.05M NaCl. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
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| Key Applications |
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| Application Notes | For use in blocking the staining of Chemicons catalog numbers AB143, AB144P and AB144. Suggested concentration 2-5 μg/mL. AG220 should be incubated with the antibody for 3 hours at +2-8°C prior to being used on the tissue sections. Enzyme is denatured by heat, but its activity level has not been determined. Residual activity may be present. Optimal working dilution must be determined by the end user. |
| Biological Information | |
|---|---|
| Concentration | 0.25 mg/mL |
| Entrez Gene Number |
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| Entrez Gene Summary | Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5 untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. SIZE: 748 amino acids; 82568 Da DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive. SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 10 µg |