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Choline Acetyltransferase, for Blocking AB143, AB144P Antibodies
Overview
Recombinant rat ChAT
Alternate Names
ChAT
Product Information
Presentation
Liquid in 50 mM KPO4 buffer pH 7.5 containing 10% glycerol and 0.05M NaCl. Contains no preservative.
Quality Level
MQ100
Applications
Key Applications
Cell Function Assay
Application Notes
For use in blocking the staining of Chemicons catalog numbers AB143, AB144P and AB144. Suggested concentration 2-5 μg/mL. AG220 should be incubated with the antibody for 3 hours at +2-8°C prior to being used on the tissue sections.
Enzyme is denatured by heat, but its activity level has not been determined. Residual activity may be present.
Optimal working dilution must be determined by the end user.
Biological Information
Concentration
0.25 mg/mL
Entrez Gene Number
NM_020549.3
NM_020984.2
NM_020985.2
NM_020986.2
Entrez Gene Summary
Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5 untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.
Gene Symbol
CHAT
ChAT
CMS1A2
CHOACTase
CMS1A
EC 2.3.1.6
UniProt Number
P28329
UniProt Summary
FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. SIZE: 748 amino acids; 82568 Da DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive. SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
