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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IHC, IP, FUNC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL1337 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-PECAM-1 Antibody, clone 390 |
| Alternate Names |
|
| Background Information | CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1) is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A Purified mouse immunoglobulin in 100mM borate buffered saline, pH 8.0, containing no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PECAM-1 Antibody, clone 390 is an antibody against PECAM-1 for use in FC, IH & IP. |
| Key Applications |
|
| Application Notes | Flow Cytometry: 1 μg/10e6 cells. Immunohistochemistry: acteone fixed frozen tissue sections Immunoprecipitation Blocking of cell adhesion: CBL1337 inhibits aggregation of L cells transfected with a variant form of CD31 (DeLisser, 1993) Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Mouse leukocyte cell line 32D |
| Clone | 390 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Specificity | CBL1337 recognizes mouse PECAM-1, a 130-140 kDa type 1 integral membrane glycoprotein. This antibody has been shown to inhibit the aggregation of L cells transfected with a variant form of PECAM-1 (DeLisser, 1993). |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P16284 # This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. SIZE: 738 amino acids; 82536 Da SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart. PTM: Phosphorylated on Ser and Tyr residues after cellular activation. SIMILARITY: SwissProt: P16284 ## Contains 6 Ig-like C2-type (immunoglobulin-like) domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 500 µg |