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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, IP, FC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL1346Z-I |
| Replaces | CBL1346Z |
| Description | Anti-mouse Integrin alphaV Antibody, clone RMV-7 (Preservative free) |
| Alternate Names |
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| Background Information | Integrin alphaV, also known as CD51, a member of the integrin family, is a 140 kD protein expressed on activated T cells, polymorphonuclear granulocytes, blastocysts, and osteoclasts. Integrin alphaV forms heterodimers by association with integrins beta1, beta3, beta5 or beta6, these complexes then act as receptors for multiple extracellular matrix proteins (ECM). The alphaV integrins have varied functions in development, stimulation/activation and homeostasis. The primary ligands for Integrin alphaV complexes are fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, and CD31. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rat monoclonal IgG1κ in buffer containing PBS without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-mouse Integrin alphaV Antibody, clone RMV-7 (Preservative free) is validated for use in Western Blotting, Immunoprecipitation, Flow Cytometry for the detection of mouse Integrin alphaV. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: A representative lot was used by an independent laboratory in LAK cell lysate. (Takahashi, K. et Al., (1990). J. Immunol. 145:4371.) Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in LAK cell lysate. (Takahashi, K. et Al., (1990). J. Immunol. 145:4371.) Blocking Assay: Adhesion and LAK cell cytotoxicity was shown using a representative lot (Takahashi, K. et Al., (1990). J. Immunol. 145:4371.) |
| Biological Information | |
|---|---|
| Immunogen | BALB/c mouse IL-2–activated killer (LAK) cells |
| Clone | RMV-7 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Specificity | Specifically recognizes Integrin alphaV, also known as CD51 or vitronectin receptor. This antibody has been reported to block binding of Integrin alphaV to vitronectin, fibronectin, fibrinogen, and CD31 in some cell types, as well as, blocking LAK cell cytotoxicity. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | ~140 kDa observed. The calculated molecular weight is 115 kDa, however Integrin alphaV has been shown as a ~140 kDa band in western blots (Takahashi, K. et Al., (1990). J. Immunol. 145:4371.). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in murine 3T3-L1 cells. Flow Cytometry Analysis: 0.2 µg of this antibody detected Integrin alphaV in murine 3T3-L1 cells . |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |