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Protein is expressed in E.coli. The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.
Product endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Description
Catalogue Number
GF324
Description
IL-1 Receptor Antagonist, Human Recombinant Animal Free
Alternate Names
IL-1
IL1RN
Background Information
Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring inflammatory inhibitor protein. It inhibits the activity of IL-1α and IL-1β by competitively blocking their binding to type I and type II receptors. IL-1Ra is produced by corneal epithelial cells, monocytes, neutrophils, macrophages, and fibroblasts. Therapeutically, IL-1Ra may help in the treatment of sepsis, cachexia, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease. Recombinant human IL-1Ra is a 17.2 kDa protein consisting of 153 amino acid residues.
Product Information
Presentation
Product is presented in 20 mM Tris, pH 8.0 and is filtered through a 0.2 micron filter before lyophilization.
Quality Level
MQ100
Biological Information
Purity
Product endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Source
Protein is expressed in E.coli. The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.
UniProt Number
P18510
Product Usage Statements
Quality Assurance
The ED50 was Determined by its ability to inhibit the IL-1α stimulation of murine D10S cell. The expected ED50 is 20-40 ng/ml in the presence of 50 pg/ml of IL-1alpha.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.
