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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ELISA, RIA, ICC | M | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB030 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-DNA Antibody, double stranded, clone BV16-13 |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Presentation | Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect DNA with Anti-DNA Antibody, double stranded, clone BV16-13 (Mouse Monoclonal Antibody), that has been shown to work in ELISA, RIA, ICC. |
| Key Applications |
|
| Application Notes | EIA/RIA Immunocytochemistry Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Epitope | double stranded, 28% reactive with single stranded |
| Clone | BV16-13 |
| Host | Mouse |
| Specificity | Double stranded DNA (dsDNyA) (B-form) from human. Shows 95% reactivity to dsDNA and 28% reactivity to ssDNA. BINDING ASSAY: Supernatant was incubated with 32P-end labeled heat-denatured (SS)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation. ANTIGEN BINDING PROFILE: Relative Binding To: Natural DNA Synthetic homopolymers dsDNA(a) ssDNA(b) poly(dA) poly(DC) poly(dG) poly(dT) poly(dI) poly(dU) 1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01 Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0). a) Native calf thymus DNA b) Heat-denatured calf thymus DNA |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 6 months. |
| Packaging Information | |
|---|---|
| Material Size | 500 µL |