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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2600 |
| Description | Anti-Glypican-1 Antibody, clone 4D1 |
| Alternate Names |
|
| Background Information | Heparan sulfate proteoglycans (HSPGs) can be divided into general categories of cell-associated forms, including syndecans, glypicans, and secreted extracellular matrix forms, such as perlecan. Glypicans contain six family members to date and are proteins bound to the outer surface of the plasma membrane by a glycosyl-phosphatidylinositol anchor and involved in cellular adhesion and migration and function as accessory molecules for signaling receptors, (Filmus, 2008 & Gui, 2006). Current publications indicate the main function of membrane-attached glypicans is to regulate the signaling of Wnts, Hedgehogs, fibroblast growth factors and bone morphogenetic proteins (BMPs), (Filmus, 2008). Glypican-1 (GPC1) is over-expressed in human gliomas and appears to have a greater capability of promoting FGF-2 signaling in some gliomas versus HSPGs in normal astrocytes. Additional observations suggest that GPC1 can impact the growth of pancreatic cancer cells by attenuating tumor angiogenesis, (Whipple, 2008). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified in 0.1 M Tris-Glycine (pH 7.4) 150 mm NaCl with 0.05% NaN3. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Glypican-1 Antibody, clone 4D1 detects level of Glypican-1 & has been published & validated for use in IH(P) & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: A 1:50 dilution of a previous lot detected strong cytoplasmic staining of tumor cells in formalin-fixed, paraffin-embedded gangliobastoma tissue sample. There was negative staining of normal, uninvolved cells. |
| Biological Information | |
|---|---|
| Immunogen | Full length recombinant protein. |
| Epitope | Full Length |
| Clone | 4D1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | MAB2600 recognizes glypican-1. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Cell surface proteoglycan that bears heparan sulfate. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor › GPI-anchor; Extracellular side. Secreted glypican-1: Secreted › extracellular space. PTM: This cell-associated glypican is further processed to give rise to a medium-released species. SEQUENCE SIMILARITIES: Belongs to the glypican family. |
| Molecular Weight | Recognizes Glypican-1 at ~ 59 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western blot on human PC-12 cell lysate using 0.5 µg/mL of Anti-Glypican-1 at 1 mg/mL. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |