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Millipore This Anti-Wipi-2, Clone 1b8.1 Antibody Is Validated For Use In Western Blotting And Immunohistochemistry (Paraffin) For The Detection Of Wipi-2. - Mill (Additional S&H or Hazmat Fees May Apply)
WD repeat domain phosphoinositide-interacting protein 2
WIPI-2
WIPI49-like protein 2
Background Information
WIPI-2, also known as Atg18, is involved in autophagy by regulating the assembly of mulitprotein complexes by presenting a 7-bladed propeller structure for interactions with phospholipids. Atg18 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. Studies show that Atg18 is recruited to early autophagosomal structural formation along with Atg16 and ULK1, and Atg18 is required for the formation of LC3-positive autophagosomes.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-WIPI-2, clone 1B8.1 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of WIPI-2.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected WIPI-2 in human pancreas tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the C-terminus of human WIPI-2.
Epitope
C-terminus
Clone
1B8.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG2aκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001028690
Gene Symbol
WIPI2
CGI-50
Purification Method
Protein G Purified
UniProt Number
Q9Y4P8
Molecular Weight
~49 kDa observed. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human testis tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected WIPI-2 in 10 µg of human testis tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
