The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Anti-Ferritin Heavy Chain Antibody, Clone 1-2.3.1.2
Millipore Anti-Ferritin Heavy Chain Antibody, Clone 1-2.3.1.2
List Price$542.08Your Price$542.08
Millipore This Anti-Ferritin Heavy Chain Antibody, Clone 1-2.3.1.2 Is Validated For Use In Western Blotting And Immunohistochemistry For The Detection Of Ferritin Heavy Chain. - Mill (Additional S&H or Hazmat Fees May Apply)
Anti-Ferritin Heavy Chain Antibody, clone 1-2.3.1.2
Alternate Names
Ferritin heavy chain
Ferritin H subunit
Cell proliferation-inducing gene 15 protein
Ferritin heavy chain, N-terminally processed
Background Information
Ferritin is a widely expressed, very large, multi-subunit, intracellular protein that stores iron and releases it in a controlled fashion. The protein is produced by almost all living organisms in some form. In humans, it acts as a buffer against iron deficiency and iron overload. Ferritin protein is a multi-subunit (24 oligomers) protein complex consisting of both large (heavy) and small (light) chains. It is primarily and intracellular protein.The subunits of Ferritin in vertebrates have an apparent molecular weight of 19 kDa or 21 kDa respectively; their sequences are about 50% homologous. When Ferritin is unbound by iron the protein is called Apoferritin. Mutations associated with the Ferritin Heavy Chain protein contribute to a syndrome called Hemochromatosis 5 which is a disorder of iron metabolism where too much iron is retained within the tissues resulting in a variety of syndromes which unfortunately manifest themselves late in life.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Ferritin Heavy Chain Antibody, clone 1-2.3.1.2 is validated for use in Western Blotting and Immunohistochemistry for the detection of Ferritin Heavy Chain.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Ferritin Heavy Chain in human bone marrow and human tonsil tissue. Immunohistochemistry Analysis: A representative lot detected Ferritin Heavy Chain in erythroid blasts of acute myeloid leukaemia M6 tissue, macrophages in the lymph node tissue, and tumor & lung-associated macrophages (Wang, W., et al. (2013). Histopahtology. 62:931–940).
Biological Information
Immunogen
Recombinant protein corresponding to human Ferritin Heavy Chain.
Clone
1-2.3.1.2
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG2aκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_002023
Gene Symbol
FTH1
FTH
FTHL6
Purification Method
Protein G Purified
UniProt Number
P02794
Molecular Weight
~21 kDa observed. Uncharacterized band may be observed at ~200 kDa.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in U251 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ferritin Heavy Chain in 10 µg of U251 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
