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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC91 |
| Description | Anti-Atg18 (WIPI-2) Antibody, clone 2A2 |
| Alternate Names |
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| Background Information | Atg18, also known as WIPI-2, is involved in autophagy by regulating the assembly of mulitprotein complexes by presenting a 7-bladed propeller structure for interactions with phospholipids. Atg18 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. Studies show that Atg18 is recruited to early autophagosomal structural formation along with Atg16 and ULK1, and Atg18 is required for the formation of LC3-positive autophagosomes. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Atg18 (WIPI-2) Antibody, clone 2A2 is validated for use in WB, IH(P) for the detection of Atg18 (WIPI-2). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Atg18 in human colorectal adenocarcinoma tissue. |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to the C-terminus of human Atg18. |
| Epitope | C-terminus |
| Clone | 2A2 |
| Host | Mouse |
| Specificity | This antibody recognizes Atg18 at the C-terminus. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et Al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008]. |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Probable early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to PtdIns3P. May bind PtdIns3P. SUBCELLULAR LOCATION: Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasmic side. Note: Enriched at preautophagosomal structure membranes in response to ptdIns3P. TISSUE SPECIFICITY: Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors. SEQUENCE SIMILARITIES: Belongs to the WD repeat SVP1 family. Contains 3 WD repeats. |
| Molecular Weight | ~49 kDa observed. 6 isoforms exist with molecular weights ranging from 42 kDa to 48 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human testis tissue lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Atg18 in 10 µg of human testis tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |