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Millipore Use Anti-Runx3 Antibody, Clone R3-5g4 (Mouse Monoclonal Antibody) Validated In Wb, Ip To Detect Runx3 Also Known As Runt-Related Transcription Factor 3, Acute Myeloid Leukemia - Mill (Additional S&H or Hazmat Fees May Apply)
Polyomavirus enhancer-binding protein 2 alpha C subunit
PEA2-alpha C
PEBP2-alpha C
SL3-3 enhancer factor 1 alpha C subunit
SL3/AKV core-binding factor alpha C subunit
Background Information
Runx3 is a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5-PYGPYGGT-3 found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer.
Product Information
Format
Purified
Control
Daudi cell lysate.
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Use Anti-Runx3 Antibody, clone R3-5G4 (mouse monoclonal antibody) validated in WB, IP to detect Runx3 also known as Runt-related transcription factor 3, Acute myeloid leukemia 2 protein, PEA2-alpha C, PEBP2-alpha C.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Runx3 in HCT116, SW620, and SW480 nuclear extracts. (Ito, K., et al. (2008). Cancer Cell. 14(3):226-237.)
Biological Information
Immunogen
Recombinant protein corresponding to human Runx3.
Clone
R3-5G4
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001026850
Gene Symbol
RUNX3
AML2
CBFA3
PEBP2A3
Purification Method
Protein G Purified
UniProt Number
Q13761
UniProt Summary
FUNCTION: CBF binds to the core site, 5-PYGPYGGT-3, of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
SUBUNIT STRUCTURE Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: The tyrosine phosphorylated form localizes to the cytoplasm.
Molecular Weight
~44 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in Daudi cell lysate.
Western Blot Analysis: 0.25 mg/mL of this antibody detected Runx3 in 10 µg Daudi cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
