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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | DB, IF, Southern Blotting, IH(P), Methylated DNA Immunoprecipitation (MeDIP), FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE146 |
| Replaces | MABE527 |
| Description | Anti-5-methylcytosine Antibody, clone 33D3 |
| Alternate Names |
|
| Background Information | 5-Methylcytosine is a modified base found in the DNA of higher eukaryotes. Methylation of DNA is known to be involved in the control of gene expression and is mediated by a class of enzymes known as DNA methyltransferases (DNMT’s). Appropriate cytosine methylation is essential for normal development and maintenance of mammalian cells. Alternations in DNA methylation patterns is often associated with cancers with the turmor DNA being hypomethylated compared to DNA from normal tissues. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1λ in buffer containing PBS and 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-5-methylcytosine Antibody, clone 33D3 (mouse monoclonal antibody) validated in DB, IF, SB, IHC(P), Flow Cytometry, MeDIP to detect 5-methylcytosine also known as methylcytosine, methylcytidine. |
| Key Applications |
|
| Application Notes | IF Habib, M., et al. (1999). Exp Cell Res. 249:46-53 IHC-P Hernadez-Blazquez, F.J., et al. (2000). Gut. 47:689-693 Flow Cytometry Reynaud, C.C., et al. (1992). Cancer Lett. 61(3):255-262 MeDIP Keshet, I., et al. (2006). Nat Genet. 38(2):149-153 |
| Biological Information | |
|---|---|
| Immunogen | Ovalbumin-conjugated 5-methylcytosine |
| Clone | 33D3 |
| Host | Mouse |
| Specificity | This antibody detects 5-Methylcytosine, but not unmethylated cytosine. |
| Isotype | IgG1λ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Predicted to react with multiple species. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein A |
| Molecular Weight | Detects methylated cytosine |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Dot Blot in methylated and unmethylated BRCA1. Dot Blot Analysis: 1 µg/mL of this antibody detected methylcytosine in methylated and unmethylated BRCA1. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |