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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE160 |
| Description | Anti-E2F4 Antibody, clone LLF4-2 |
| Alternate Names |
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| Background Information | E2F4 is the most common E2F family member in a number of cell types. E2F4 deficiency has been shown to cause a decrease in the thickness of the gut epithelium, decrease in maturation of hematopoietic cell types and an increased rate of apoptosis, suggesting that E2F4 plays a role in normal maturation of several cell types. E2F4 has also been shown to be essential for the normal development of airway epithelium and early eye development. Other data has implicated E2F4 during normal heart development, specifically cardiomyocyte proliferation. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-E2F4 Antibody, clone LLF4-2 is a Mouse Monoclonal Antibody for detection of E2F4 also known as Transcription factor E2F4, E2F-4 & has been validated in WB & IHC. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Kinross, K., et al. (2006). Blood. 108(3):886-895.) |
| Biological Information | |
|---|---|
| Immunogen | Histidine-tagged recombinant protein corresponding to human E2F4. |
| Clone | LLF4-2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq]. |
| Gene Symbol |
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| Purification Method | Protein G |
| UniProt Number |
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| UniProt Summary | FUNCTION: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5-TTTC[CG]CGC-3 found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein. SUBUNIT STRUCTURE: Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. The E2F4/DP-1 dimer interacts preferentially with pocket protein RBL1, which inhibits the E2F transactivation domain. Lower affinity interaction has been found with retinoblastoma protein RB1. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Interacts with HCFC1. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. DEVELOPMENTAL STAGE: Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle. PTM: Differentially phosphorylated in vivo. POLYMORPHISM: The poly-Ser region of E2F4 is polymorphic and the number of Ser varies in the population (from 8 to 17). The variation might be associated with tumorigenesis. SEQUENCE SIMILARITIES: Belongs to the E2F/DP family. |
| Molecular Weight | Bands may be detected at ~45 and ~60 kDA |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected E2F4 on 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |