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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE164 |
| Description | Anti-ID4 Antibody, clone DML07 |
| Alternate Names |
|
| Background Information | ID4 is an inhibitor of differentiation protein, which, along with ID1, ID2 and ID3, plays a key role in the regulation of lineage commitment, cell fate decisions and in the timing of differentiation during neurogenesis, lymphopoiesis and neovascularisation (angiogenesis). ID proteins, induced by the bone morphogenic protein-SMAD pathway, have also been shown to cooperate with LIF to ensure that embryonic stem cells opt for self-renewal. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-ID4 Antibody, clone DML07 (Mouse Monoclonal Antibody) validated in WB to detect ID4 also known as DNA-binding protein inhibitor ID-4, Class B basic helix-loop-helix protein 27, bHLHb27. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 1 µg/mL from a representative lot detected ID4 on 10 µg of mouse lymph node tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human ID4. |
| Clone | DML07 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 [PubMed 7665172]).[supplied by OMIM]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. SUBUNIT STRUCTURE: Heterodimer with other HLH proteins. SUBCELLULAR LOCATION: Nucleus. SEQUENCE SIMILARITIES: Contains 1 basic helix-loop-helix (bHLH) domain. |
| Molecular Weight | ~17 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human lung tissue lysate. Western Blot Analysis: 1 µg/mL of this antibody detected ID4 on 10 µg of human lung tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |