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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC | M | AlexaFluor®647 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE263-AF647 |
| Description | Anti-unmethylated Histone H3 (Lys9) Antibody, clone 9B1-2G6, Alexa Fluor® 647 Conjugate |
| Alternate Names |
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| Background Information | Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the beads on a string structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2. While lysine demethylation is mediated by enzymes such as JmjC domain-containing proteins. |
| Product Information | |
|---|---|
| Format | AlexaFluor®647 |
| Presentation | Purified conjugated mouse monoclonal antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide. |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | This Anti-unmethylated Histone H3 (Lys9) Antibody, clone 9B1-2G6 is validated for use in Immunocytochemistry for the detection of unmethylated Histone H3 (Lys9). |
| Key Applications |
|
| Application Notes | See data sheet for Cat. No. MABE263, Anti-unmethylated Histone H3 (Lys9), clone 9B1-2G6, for additional Western blot and dot blot specificity analysis performed using an unconjugated version of this antibody. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human unmethylated Histone H3 (Lys9). |
| Clone | 9B1-2G6 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | This antibody recognizes Histone H3 unmethylated at Lys9. This antibody does not cross react with methyl-Histone (Lys29). This antibody detects unmethylated Histone H3 (Lys9) when Ser10 is phosphorylated. |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~15 kDa observed. Molecular weight was observed in parent product MABE263 |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected unmethylated Histone H3 (Lys9) in HeLa cells. Alexa Fluor® is a registered trademark of Life Technologies®. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |