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Millipore This Anti-Hexim 1 Antibody, Clone 10d2.1 Is Validated For Use In Western Blotting And Immunohistochemistry (Paraffin) For The Detection Of Hexim 1. - Mill (Additional S&H or Hazmat Fees May Apply)
Hexim1 is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. Hexim1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. Hexim1 may also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. The 7SK snRNP complex is composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), Hexim1, Hexim2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Hexim1 also interacts with the N-CoR complex through NCOR1, ESR1, NR3C1, and may interact with NF-kappa-B through REL A. Hexim1 is located in the nucleus where it can bind with alpha-importin, and can also be found in the cytoplasm. Hexim1 is up-regulated by HMBA (hexamethylene bisacetamide) at the protein level and down-regulated by estrogen.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Hexim 1 Antibody, clone 10D2.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Hexim 1.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Hexim 1 in human placenta tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the C-terminus of human Hexim 1.
Epitope
C-terminus
Clone
10D2.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG2bκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_006451
Gene Symbol
HEXIM1
CLP1
EDG1
HIS1
MAQ1
Purification Method
Protein G Purified
UniProt Number
O94992
Molecular Weight
~60 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human placenta tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Hexim 1 in 10 µg of human placenta tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
