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Millipore This Anti-Aiolos Antibody, Clone 9d10 Is Validated For Use In Western Blotting And Chip-Seq And Chromatin Immunoprecipitation (Chip) For The Detection Of Aiolos. - Mill (Additional S&H or Hazmat Fees May Apply)
Aiolos is a zinc finger DNA binding transcription factor important in lymphocyte differentiation and development. Aiolos plays an essential role in the regulation of B-cell differentiation including, proliferation and maturation to plasma cells. Aiolos also works as a cofactor in gene suppression via its interaction with the transcription factor FOXP3 in regulatory T cells as well, and Aiolos is involved in various chromatin functions too through its interaction with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A. Aiolos is localized in the nucleus primarily but can be found in the cytoplasm depending upon the cellular situation. Aiolos is expressed in leukocytes and found in high concentration in spleen and thymus and has multiple isoforms. Activity is regulated via phosphorylation.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Aiolos Antibody, clone 9D10 is validated for use in Western Blotting and ChIP-seq and Chromatin Immunoprecipitation (ChIP) for the detection of Aiolos.
Key Applications
Western Blotting
ChIP-seq
Chromatin Immunoprecipitation (ChIP)
Application Notes
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012).
ChIP-seq: A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012).
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Aliolos in an HL-60 cell lysate
Biological Information
Immunogen
Recombinant protein corresponding to human Aiolos.
Clone
9D10
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG2bκ
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001244337
Gene Symbol
IKZF3
ZNFN1A3
Purification Method
Protein G Purified
UniProt Number
Q9UKT9
Molecular Weight
~ 58-70 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in a Jurkat cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Aliolos in 10 µg of a Jurkat cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
