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Millipore This Anti-E3 Ubiquitin-Protein Ligase Uhrf1 Antibody, Clone 7c8 Is Validated For Use In Immunocytochemistry And Chromatin Immunoprecipitation (Chip) And Western Blotting - Mill (Additional S&H or Hazmat Fees May Apply)
Ubiquitin-like PHD and RING finger domain-containing protein 1
hUHRF1
Ubiquitin-like-containing PHD and RING finger domains protein 1
Background Information
Ubiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8 is validated for use in Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and Western Blotting for the detection of E3 ubiquitin-protein ligase UHRF1.
Key Applications
Immunocytochemistry
Chromatin Immunoprecipitation (ChIP)
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected E3 ubiquitin-protein ligase UHRF1 in overexpressed lysate (Martin Walsh, Mount Sinai School of Medicine). Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated E3 ubiquitin-protein ligase UHRF1 in NTera2 cells (Martin Walsh, Mount Sinai School of Medicine).
Biological Information
Immunogen
Recombinant protein corresponding to human E3 ubiquitin-protein ligase UHRF1.
Clone
7C8
Concentration
1 mg/mL
Host
Mouse
Isotype
IgG2bκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001041666
Gene Symbol
UHRF1
ICBP90
NP95
RNF106
Purification Method
Protein G Purified
UniProt Number
Q96T88
Molecular Weight
90 kDa calculated
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected E3 ubiquitin-protein ligase UHRF1 in HeLa cells. Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
