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Millipore This Anti-E3 Ubiquitin-Protein Ligase Uhrf1, Clone Bf18.11 Antibody Is Validated For Use In Western Blotting And Immunocytochemistry And Immunoprecipitation For The Detection - Mill (Additional S&H or Hazmat Fees May Apply)
Ubiquitin-like PHD and RING finger domain-containing protein 1
mUhrf1
Ubiquitin-like-containing PHD and RING finger domains protein 1
Background Information
Ubiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-E3 ubiquitin-protein ligase UHRF1, clone BF18.11 Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunoprecipitation for the detection of E3 ubiquitin-protein ligase UHRF1.
Key Applications
Western Blotting
Immunocytochemistry
Immunoprecipitation
Application Notes
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected E3 ubiquitin-protein ligase UHRF1 in NIH/3T3 cells. Immunoprecipitation Analysis: A representative lot immunoprecipitated E3 ubiquitin-protein ligase UHRF1 in 5 mg of NIH/3T3 cell lysate (Roberto Papait, FIRC Institute of Molecolar Oncology).
Biological Information
Immunogen
His-tagged recombinant protein corresponding to mouse E3 ubiquitin-protein ligase UHRF1.
Clone
BF18.11
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001104548
Gene Symbol
Uhrf1
Np95
Purification Method
Protein G Purified
UniProt Number
Q8VDF2
Molecular Weight
~88 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in NIH-3T3 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected E3 ubiquitin-protein ligase UHRF1 in 10 µg of NIH-3T3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
