The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore This Anti-Rbp-Jk, Clone 1f1 Antibody Is Validated For Use In Immunoprecipitation And Chromatin Immunoprecipitation (Chip) And Electrophoretic Mobility Shift Assay For The Detection Of Rbp-Jk. - Mill (Additional S&H or Hazmat Fees May Apply)
Recombining binding protein suppressor of hairless
CBF-1
J kappa-recombination signal-binding protein
RBP-J kappa
RBP-J
RBP-JK
Renal carcinoma antigen NY-REN-30
Background Information
RBP-jk, Recombinant signal binding protein for immunoglobin kappa J region, is a human homolog for the Drosophila gene, suppressor of hairless. RBP-jk is located in the nucleus and is a 60 kDa monomeric protein that is used as a promoter region in notch signaling. Notch signaling is important in cell to cell communication which allows "a differentiating cell to suppress the same differentiation fate in a neighboring cell (the receiving cell). This ensures that the correct number of cells are distributed to distinct cell fates." When RBP-jk is not associated with Notch proteins, it acts as a transcriptional repressor. A disease associated with RBP-jk is the Epstein Barr virus.
Product Information
Format
Purified
Presentation
Purified rat IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-RBP-Jk, clone 1F1 Antibody is validated for use in Immunoprecipitation and Chromatin Immunoprecipitation (ChIP) and Electrophoretic Mobility Shift Assay for the detection of RBP-Jk.
Key Applications
Immunoprecipitation
Chromatin Immunoprecipitation (ChIP)
Electrophoretic Mobility Shift Assay
Application Notes
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using Lymphoblastoid cell line.
Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using DG75 cells.
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using Hippocampal NSCs (Ehm, O., et al. (2010). Journal of Neuroscience. 30(41):13794-18707).
Electrophoretic Mobility Shift Assay: A representative lot detected RBP-Jk using Hippocampal lysate (Ehm, O., et al. (2010). Journal of Neuroscience. 30(41):13794-18707).
Biological Information
Immunogen
Recombinant protein corresponding to human RBP-Jk.
Clone
1F1
Concentration
Please refer to lot specific datasheet.
Host
Rat
Isotype
IgG2aκ
Species Reactivity
Human
Drosophila
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_005340
Gene Symbol
RBPJ
IGKJRB
IGKJRB1
RBPJK
RBPSUH
Purification Method
Protein G Purified
UniProt Number
Q06330
Molecular Weight
~55 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Immunoprecipitation in HeLa cell lysate.
Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated RBP-Jk in 0.5 mg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
