The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore This Anti- Sting, Clone 41 Antibody Is Validated For Use In Western Blotting And Flow Cytometry For The Detection Of Sting. - Mill (Additional S&H or Hazmat Fees May Apply)
STING, also known as mSTING, or Endoplasmic reticulum interferon stimulator, ERIS, Mediator of IRF3 activation, MMITA or Transmembrane protein 173, and encoded by the gene Tmem 173/Eris/Mita/Mpys/Sting, is a facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferons. STING acts by recognizing and binding cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, and cyclic GMP-AMP (cGAMP), a messenger produced in response to DNA virus in the cytosol. Upon binding STING is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state. STING also mediates cell death signaling via the activation of the ERK pathway. STING is found in multiple intracellular locations including the endoplasmic reticulum, plasma cell membrane, mitochondrion outer membrane, and perinuclear regions. STING is expressed in the B-cell lineage both immature and mature B cells but not plasma cells. Mutations in STING cause defects in innate immunity and compromised immune health.
Product Information
Format
Purified
Presentation
Purified rat monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti- STING, clone 41 Antibody is validated for use in Western Blotting and Flow Cytometry for the detection of STING.
Key Applications
Western Blotting
Flow Cytometry
Application Notes
Flow Cytometry Analysis: 2 µg of this antibody detected STING in mouse splenocyte cells.
Biological Information
Immunogen
Recombinant protein corresponding to the cytoplasmic domain of mouse STING.
Epitope
Cytoplasmic domain
Clone
41
Concentration
0.5 mg/mL
Host
Rat
Isotype
IgMκ
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_082537
Gene Symbol
Tmem173
Eris Mita
Mpys
Sting
UniProt Number
Q3TBT3
Molecular Weight
~42 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at ~38 kDa and ~48 kDa.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in RAW264.7 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected STING in 10 µg of RAW264.7 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
