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Millipore Detect Spred-1 Using This Mouse Monoclonal Antibody, Anti-Spred-1 Antibody, Clone M23-P2g3 Validated For Use In Western Blotting & Icc. - Mill (Additional S&H or Hazmat Fees May Apply)
Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) is a tyrosine kinase substrate which has a cysteine-rich domain which resembles the SPR domain at the carboxy terminus. SPRED1 associated with Ras blocks phosphorylation of Raf and disrupts MAP kinase signaling during the differentiation of neuronal cells and myocytes. A mutation in SPRED1 has been linked to Lugius syndrome, an autosomal dominant RAS-MAPK pathway syndrome.
Product Information
Format
Affinity Purified
Presentation
Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Spred-1 using this mouse monoclonal antibody, Anti-Spred-1 Antibody, clone M23-P2G3 validated for use in western blotting & ICC.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A representative lot detected Spred-1 in HeLa cells.
Biological Information
Immunogen
Linear peptide corresponding to human Spred-1.
Clone
M23-P2G3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1λ
Species Reactivity
Mouse
Rat
Species Reactivity Note
Mouse, Rat. Predicted to react with Human based on 100% sequence homology.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_689807
Gene Symbol
SPRED1
Purification Method
Protein g Purified
UniProt Number
Q7Z699
Molecular Weight
~ 60 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Spred-1 in 10 µg of mouse brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
