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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS64 |
| Description | Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2 |
| Alternate Names |
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| Background Information | The p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect phospho-p38 (Thr180/Tyr182) using this Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2 validated for use in WB & IC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected p38 in Anisomycin treated NIH/3T3 cells. |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to human p38 phosphorylated at Thr180 and Tyr182. |
| Epitope | Phosphorylated Thr180 and Tyr182 |
| Clone | 6E5.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes p38 when phosphorylated at Thr180 and Tyr182. |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Responds to activation by environmental stress, pro- inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis. COFACTOR: Magnesium. SIZE: 360 amino acids; 41293 Da SUBUNIT: Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR. This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation. Interacts with SPAG9 (by similarity). Interacts with NP60. SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). TISSUE SPECIFICITY: Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.DOMAIN:SwissProt: Q16539 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme. & Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 41 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in Anisomycin treated and untreated HeLa cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected p38 on 10 µg of Anisomycin treated and untreated HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |