Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, FC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT134 |
| Replaces | MAB1989 |
| Description | Anti-VE-cadherin Antibody, clone BV6 |
| Alternate Names |
|
| Background Information | Human Vascular Endothelial (VE)-cadherin is a calcium-dependent adhesion molecule strictly located at cell-to-cell junctions. VE-cadherin is present in all types of endothelium (veins, arteries, capillary and large vessels). Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. VE-cadherin also associates with alpha-catenin forming a link to the cytoskeleton. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-VE-cadherin Antibody, clone BV6 is validated for use in WB, IC, FC, IH(P) for the detection of VE-cadherin. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected VE-cadherin in the cell-cell junctions of HUVEC cells. Immunohistochemistry Analysis: A 1:10-1:20 dilution of a representative lot was used by an independent laboratory in paraffin-embedded tissue sections. Buffered formalin fixation is recommended with a fixation period of no longer than 12 hours. High heat antigen retrieval in citrate buffer (Cat. No. 21545) is also suggested. Antibody can also be used to label acetone-fixed cryostat sections or cells using a immunoperoxidase staining protocol (eg. IHC Select® Detection Kit, Cat. No. DAB150) Flow Cytometry Analysis: A representative lot was used in the presence of Ca2+. Note: Use PBS + 2-5mM EDTA for cell detachment. Western Blot Analysis: Non-reducing conditions may be needed, Ca2+ required in buffer (2-5 mm). |
| Biological Information | |
|---|---|
| Immunogen | HUVEC cells |
| Clone | BV6 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. SUBUNIT STRUCTURE: Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. SUBCELLULAR LOCATION: Cell junction. Cell membrane; Single-pass type I membrane protein. Note: Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions. |
| Molecular Weight | ~120 kDa observed. The calculated molecular weight is 82 kDa but it will run between ~90-140 kDa because this protein is glycosolated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HUVEC cell lysate. Western Blot Anlaysis: A 1:2,000 dilution of this antibody detected VE-cadherin in HUVEC cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |