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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT182 |
| Description | Anti-Rab5A Antibody, clone 7D8.1 |
| Alternate Names |
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| Background Information | Rab5A is a membrane associated Ras related GTPase that is required for endosome fusion with the plasma membrane. Rab5A also contributes to filopodia extension and other cellular cytoplasmic extensions. Rab5A plays important roles in cellular differentiation, neurogenesis, exocytosis and protein transport. Rab proteins are a large family of GTP-binding proteins that regulate cellular membrane traffic and organelle identity. When membrane bound, each Rab has a very specific cellular location and recognizes distinct subsets of intracellular membranes, which allows Rab proteins to act as as key cellular regulators, determining the recruitment of downstream effectors to the correct membrane at the correct time. Recent research indicates that the localization of Rab proteins happens by sensing the stored curvature elastic energy in the membrane and the specific RabGEFs (Rab-activating guanosine diphosphate/guanosine triphosphate exchange factors) that associate with a particular Rab. Rab5A associates with Rabex-5 and this association provides the determinants for Rab5A specific membrane targets. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | This Anti-Rab5A Antibody, clone 7D8.1 is validated for use in Western Blotting and Immunohistochemistry for the detection of Rab5A. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Rab5A in human testis tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Rab5A. |
| Clone | 7D8.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | ~24 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK-293 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Rab5A in 10 µg of HEK-293 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |